After repeated test, formula improvement and research summary, DNFeed 2 + DNFeed B2, a high-performance culture medium for CHO cells, was officially launched recently.
DN feed 2 supplement is developed for Chinese hamster ovary(CHO) cell culture in bioprocessing applications. DN feed 2 is an animal-derived component free(ACF), protein free (PF), chemically defined (CD).
DN feed 2 is suitable for general therapeutic recombinant protein products manufacturing in feb-batch culture on industrial CHO cell lineages (CHO-K1, CHO-S, DuxB11, DG44 and CHOZN GS).
DN feed 2 provides nutrients such as amino acids, vitamins, salts, trace elements et al. DN feed 2 doesn’t contain any growth factor(such as insulin and IGF) and hydro-lysates, ensuring batch to batch consistency and increasing cell culture process efficiency. DN feed 2 doesn't contain hypoxanthine, thymidine and L-glutamine, suitable for GS selection and dhfr selection.
The figure shows the cell growth and expression data of DN feed 2 + DN feed B2 compared with the imported supplement.
DN feed 2 and DN feed 2B are intended for use together in defined concentration.
The supplement should be added to the cultivation vessel as individual solution and should not be mixed in advance otherwise will cause precipitation. The recommended ratio of DN feed 2 to DN feed 2B is 10 to 1(v/v). The total amount and regime of feed will need to be adjusted according to the nutritional requirements of each specific clone.
① DN feed 2 supplement has a pH close to neutral and contains 37.5g/L glucose.
DN feed 2B supplements has an alkaline pH and is a concentrated solution of amino acids.
② It's recommended to initate feeding after cell reaching mid to late exponential phase（usually viable cell density reach to 2.0~3.0×106 cells/mL）.The recommend feed strategy as following:
|Time||DN Feed 2+DN Feed B2|
|Day 3||3% + 0.3%|
|Day 5||3% + 0.3%|
|Day 7||3% + 0.3%|
|Day 9||3% + 0.3%|
|Day 11||3% + 0.3%|