Maxgrow Supplement is a kind of culture medium additive without animal origin, protein composition and chemical composition limitation. It is suitable for the use of Chinese hamster ovary cells (CHO) for cloning and screening, batch culture, feeding batch culture and perfusion culture in the development and production of therapeutic protein products. The addition of Maxgrow Supplement was suitable for the culture of CHOK1, CHOK1SV, CHOS and other cell lines by GS and DHFR screening systems.
In the monoclonal screening process, the appropriate proportion of Maxgrow Supplement could reduce the amount of serum/serum substitute used and increase the clone formation rate. In the development and production of CHO cell culture technology, the appropriate proportion of Maxgrow Supplement can significantly increase the growth density of difficult-growing CHO cells/clones, improve the vitality and improve the production performance.
Application 1: improve the monoclonal formation rate of CHO cells
Case1: CHOK1 Pool monoclonal formation rate increased by 14.5%
Case2: CHOK1 Subclone monoclone formation rate increased by 51.4%
Case1: the highest cell density of CHOK1 cell item 1 was increased by 64.1%, and the expression of antibody was increased by 21.9%
Case2: the highest cell density of CHOK1 cell item 2 was increased by 20.9%, and the expression of antibody was increased by 23.4%
Case3: the highest cell density of CHOK1 cell item 3 was increased by 57.1%, and the expression of antibody was increased by 43.0%