AR8+BR8 CHO super-concentrated high-efficiency feed is a high-efficiency feeding medium that is animal-derived-component-free (ADCF), protein-free and chemical-limited (CD), which is suitable for fed-batch culture in the process of developing and producing therapeutic protein products using Chinese hamster ovary (CHO) cells. This product does not contain growth factor, hypoxanthine, thymine and L-glutamine and is suitable for the culture of CHO-K1, CHO-S, DG44 and other cell lines using GS and dhfr screening systems.
AR8 CHO super-concentrated high-efficiency feed should be used in conjunction with BR8 CHO super-concentrated high-efficiency feed at the same time.
AR8+BR8 CHO super-concentrated high-efficiency feed is a high-efficiency feeding formula designed to improve the growth and production performance of CHO cells, which is rich in nutrients such as amino acids and vitamins, and can meet the nutritional needs of cell culture in high density and high expression of products. Combining with characteristics of cell lines, reasonable feeding strategy can significantly improve cell growth and antibody expression performance.
AR8 CHO super-concentrated high-efficiency feed: neutral pH and glucose level of 90g/L;
BR8 CHO super-concentrated high-efficiency feed: alkaline pH and no glucose.
It is recommended to carry out the first feeding at the middle and late phases of cell exponential growth, which usually starts at a cell density of more than 2.0-3.0×106 cells/mL at Day 3. The recommended feeding proposal is described below and the optimized ratio can be customized according to the cell line used:
AR8 CHO Super-concentrated High-efficiency Feed
AR8 CHO Super-concentrated High-efficiency Feeding Medium
BR8 CHO Super-concentrated High-efficiency Feed
BR8 CHO Super-concentrated High-efficiency Feeding Medium