• AR8 + BR8

AR8 + BR8

Product Description

AR8+BR8 CHO super-concentrated high-efficiency feed is a high-efficiency feeding medium that is animal-derived-component-free (ADCF), protein-free and chemical-limited (CD), which is suitable for fed-batch culture in the process of developing and producing therapeutic protein products using Chinese hamster ovary (CHO) cells. This product does not contain growth factor, hypoxanthine, thymine and L-glutamine and is suitable for the culture of CHO-K1, CHO-S, DG44 and other cell lines using GS and dhfr screening systems.

AR8 CHO super-concentrated high-efficiency feed should be used in conjunction with BR8 CHO super-concentrated high-efficiency feed at the same time.


User Manual

AR8+BR8 CHO super-concentrated high-efficiency feed is a high-efficiency feeding formula designed to improve the growth and production performance of CHO cells, which is rich in nutrients such as amino acids and vitamins, and can meet the nutritional needs of cell culture in high density and high expression of products. Combining with characteristics of cell lines, reasonable feeding strategy can significantly improve cell growth and antibody expression performance.

  • AR8 CHO super-concentrated high-efficiency feed: neutral pH and glucose level of 90g/L;

    BR8 CHO super-concentrated high-efficiency feed: alkaline pH and no glucose.

  • It is recommended to carry out the first feeding at the middle and late phases of cell exponential growth, which usually starts at a cell density of more than 2.0-3.0×106 cells/mL at Day 3. The recommended feeding proposal is described below and the optimized ratio can be customized according to the cell line used:

    Feeding Time

    AR8+BR8

    Day 3

    2%+0.5%

    Day 5

    2%+0.5%

    Day 7

    4%+1.0%

    Day 9

    4%+1.0%

    Day 11

    2%+0.5%

    Day 13

    2%+0.5%


Product Catalogue

Name

Article No.

Package

Specification

AR8 CHO Super-concentrated   High-efficiency Feed

F0110-002

Liquid

1L

AR8 CHO Super-concentrated   High-efficiency Feeding Medium

FP010-001

Powder

100g

FP010-010

Powder

1kg

FP010-050

Powder

5kg

BR8 CHO Super-concentrated   High-efficiency Feed

F011-002

Liquid

1L

BR8 CHO Super-concentrated   High-efficiency Feeding Medium

FP011-001

Powder

100g

FP011-010

Powder

1kg

FP011-050

Powder

5kf


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