DN feed 1 is a high-efficiency feeding medium that is animal-derived-component-free (ADCF), protein-free and chemical-limited (CD), which is suitable for fed-batch culture in the process of developing and producing therapeutic protein products using Chinese hamster ovary (CHO) cells. This product does not contain growth factor, hypoxanthine, thymine and L-glutamine and is suitable for the culture of CHO-K1, CHO-S, DG44, CHOZN and other cell lines using GS and dhfr screening systems.
DN feed 1 should be used in conjunction with DN feed B at the same time.
DN feed 1 is a high-efficiency feeding formula designed to improve the growth and production performance of CHO cells, which is rich in nutrients such as amino acids and vitamins, and can meet the nutritional needs of cell culture in high density and high expression of products. Combining with characteristics of cell lines, reasonable feeding strategy can significantly improve cell growth and antibody expression performance.
DN feed 1 liquid medium: neutral pH and glucose level of 37.5g/L;
DN feed B liquid medium: alkaline pH and no glucose.
It is recommended to carry out the first feeding at the middle and late phases of cell exponential growth, which usually starts at a cell density of more than 2.0-3.0×106 cells/mL at Day 3. The recommended feeding proposal is described below and the optimized ratio can be customized according to the cell line used:
DN feed 1+DN feed B
DN feed 1
DN feed B