• DN feed2+DN feed B2

DN feed2+DN feed B2

Product Description

DN feed 2 is a high-efficiency feeding medium that is animal-derived-component-free (ADCF), protein-free and chemical-limited (CD), which is suitable for fed-batch culture in the process of developing and producing therapeutic protein products using Chinese hamster ovary (CHO) cells. This product does not contain growth factor, hypoxanthine, thymine and L-glutamine and is suitable for the culture of CHO-K1, CHO-S, DG44, CHOZN and other cell lines using GS and dhfr screening systems.

DN feed 2 should be used in conjunction with DN feed B2 liquid at the same time.


User Manual

DN feed 2 is a high-efficiency feeding formula designed to improve the growth and production performance of CHO cells, which is rich in nutrients such as amino acids and vitamins, and can meet the nutritional needs of cell culture in high density and high expression of products. Combining with characteristics of cell lines, reasonable feeding strategy can significantly improve cell growth and antibody expression performance.

  • DN feed 2 medium: neutral pH, no glucose for powder package and glucose level of 75g/L for liquid package;

    DN feed B2 medium: alkaline pH and no glucose.

  • It is recommended to carry out the first feeding at the middle and late phases of cell exponential growth, which usually starts at a cell density of more than 2.0-3.0×106 cells/mL at Day 3. The recommended feeding proposal is described below and the optimized ratio can be customized according to the cell line used:


    Feeding Time

    DN feed 2+DN feed B2

    Day 3

    3%+0.3%

    Day 5

    3%+0.3%

    Day 7

    3%+0.3%

    Day 9

    3%+0.3%

    Day 11

    3%+0.3%


Notes

  • Hypoxanthine and thymine can be added as needed for cell line culture using GS screening system;

  • 3~8mM L-glutamine can be added as needed for cell line culture using dhfr screening system


Product Catalogue

Feeding Medium

Article No.

Package

Specification

Prepared Content

DN feed   2

FT002-1L

Liquid

1L

1L

DN feed   2 Medium

FP002-100g

Powder

100g

101.62g/L

DN feed   2 Medium

FP002-1kg

Powder

1kg

DN feed   2 Medium

FP002-5kg

Powder

5kg

DN feed   B2

FT003-300ml

Liquid

300ml

300ml

DN feed   B2 Medium

FP003-100g

Powder

100g

100.70g/L

DN feed   B2 Medium

FP003-1kg

Powder

1kg

 



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