2×103A is a high-efficiency feeding medium that is animal-derived-component-free (ADCF) and protein-free, which is suitable for fed-batch culture in the process of developing and producing therapeutic protein products using Chinese hamster ovary (CHO) cells. This product does not contain hypoxanthine, thymine and L-glutamine, which has been widely used in the culture of CHO-K1, CHO-S, DG44, CHOZN and other cell lines using GS and dhfr screening systems.
2×103A is a high-efficiency feeding formula designed to improve the growth and production performance of CHO cells, which is rich in nutrients such as amino acids and vitamins, and can meet the nutritional needs of cell culture in high density and high expression of products. Combining with characteristics of cell lines, reasonable feeding strategy can significantly improve cell growth and antibody expression performance.
Recommended Feeding Strategy:
2.96g/L high-efficiency feeding medium B is required to be added in the preparation process
It is recommended to carry out the first feeding at the middle and late phases of cell exponential growth, which usually starts at a cell density of more than 2.0×106 cells/mL. The recommended feeding proposal is described below and the optimized ratio can be customized according to the cell line used:
2×103A feed can be used in combination with BR8 to further improve the culture efficiency, and the concentration of BR8 is recommended at 0.5% per addition.
|Feeding Time||2 x 103A||2 x 103A+BR8|
|2×103 CHO High-efficiency Feed A||F103-201.A||Liquid||1L|
|2×103A High-efficiency Feeding Medium A||FP103A-001||Powder||100g|
|High-efficiency Feeding Medium B||BP100-001||Powder||10g|