Media C is a basic medium that is animal-derived-component-free (ADCF), serum-free and chemical-limited, which is suitable for batch culture, fed-batch culture and perfusion culture in the process of developing and producing therapeutic protein products using Chinese hamster ovary (CHO) cells. Media C does not contain growth factor, hypoxanthine, thymine and L-glutamine, which is suitable for culture of CHO-K1, CHO-S, DG44 and other cell lines using GS and dhfr screening systems.
Media C is designed to improve the growth and production performance of CHO cells.
This product contains no anti-agglomeration agents, if there is an agglomeration, it is recommended to add 0.1~1.0g/L dextran sulfate or 1× Anti-Clumping Agent (GIBCO Article No. 0010057DG).
2.22g/L sodium bicarbonate and 1.80g/L P188 can be added for powder package
If insulin is added, a concentration of 0.5-10mg/L is recommended
Hypoxanthine and thymine can be added as needed for cell culture using GS screening system
2~8mM L-glutamine can be added as needed for cell culture using dhfr screening system