S3 CD CHO medium is a basic medium that is animal-derived-component-free (ADCF), serum-free and chemical-limited, which is suitable for batch culture, fed-batch culture and perfusion culture in the process of developing and producing therapeutic protein products using Chinese hamster ovary (CHO) cells. S3 CD CHO medium does not contain growth factor, hypoxanthine, thymine and L-glutamine, which is suitable for culture of CHO-K1, CHO-S, DG44 and other cell lines using GS and dhfr screening systems.
S3 CHO medium is designed to improve the growth and production performance of CHO cells.
This product contains no anti-agglomeration agents, if there is an agglomeration, it is recommended to add 0.1~1.0g/L dextran sulfate or 1× Anti-Clumping Agent (GIBCO Article No. 0010057DG).
Hypoxanthine and thymine can be added as needed for cell culture using GS screening system.
2~8mM L-glutamine can be added as needed for cell culture using dhfr screening system.
2.1g/L sodium bicarbonate and 2.96g/L basic medium B can be added for powder package.
If insulin is added, a concentration of 0.5-10 mg/L is recommended.
5g/L yeast hydrolysate is added as needed for S3 CHO medium.